p nf2  (Danaher Inc)

Journal: Microorganisms

Article Title: Phosphoproteome Reveals the Role of Baicalin in Alleviating rPVL-Induced Cell Cycle Arrest in BMECs

doi: 10.3390/microorganisms13071673

Figure Lengend Snippet: rPVL inhibited the phosphorylation of BCLAF1 S285 , CDK7 T170 , NF2 S518 , and PKM2 S37 , whereas baicalin reversed the hypophosphorylation of these cell-cycle-related proteins induced by rPVL. ( A – D ) Representative immunofluorescence images of P-BCLAF1 S285 /P-CDK7 T170 /P-NF2 S518 /P-PKM2 S37 . ( E – H ) Quantification of immunofluorescence images. The results indicated that after rPVL treatment, the fluorescence intensity of the phosphorylated proteins described above decreased. However, when baicalin was administered prior to rPVL exposure, the inhibitory effect of rPVL on the phosphorylation of these cell-cycle-related proteins decreased, leading to an increase in fluorescence intensity. ( I ) The expression of cell-cycle-related proteins in BMECs was analyzed through Western blotting. ( J – M ) Western blot analysis revealed that the phosphorylation levels of BCLAF1 S285 , CDK7 T170 , NF2 S518 , and PKM2 S37 decreased following rPVL treatment but increased after baicalin intervention. The asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001) indicate significant differences between groups.

Article Snippet: NF2 (1:7000, Cat. No. 21686–1-AP), P-NF2 (Ser518, 1:7000, Cat. No. 28851–1-AP), CDK7 (1:2800, Cat. No. 27027–1-AP), and PKM (1:2800, Cat. No. 10078–2-AP) antibodies were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Phospho-proteomics, Immunofluorescence, Fluorescence, Expressing, Western Blot

Journal: Microorganisms

Article Title: Phosphoproteome Reveals the Role of Baicalin in Alleviating rPVL-Induced Cell Cycle Arrest in BMECs

doi: 10.3390/microorganisms13071673

Figure Lengend Snippet: Preemptive intervention with baicalin alleviated the pathological damage to mouse mammary tissue caused by rPVL and reversed the hypophosphorylation of cell cycle regulatory proteins induced by rPVL in the mammary tissue. ( A – F ) Histopathology of mammary tissue after infection with S. aureus and rPVL (400×). ( A ) Control (blank). ( B ) Control (0.9% NaCl). ( C ) S. aureus ATCC49775. ( D ) rPVL. ( E ) Bai+ S. aureus ATCC49775. ( F ) Bai+rPVL. The baicalin groups were administered 100 mg/kg baicalin. Arrows indicate inflammatory cells, triangles indicate macrophages, thick arrows point to eosinophils, and pentagrams highlight areas of papillary wall edema and thickening. ( K ) Representative immunohistochemical images of BCLAF1 S285 , CDK7 T170 , NF2 S518 , and PKM2 S37 (400×). ( G – J ) The bar charts show the percentages of DAB-positive tissues. The asterisks and well numbers (* p < 0.05, ** p < 0.01, # p < 0.05, *** p < 0.001, ## p < 0.01, #### p < 0.0001 and ns p > 0.05) indicate significant changes in the expression of the above cell-cycle-regulating proteins in the rPVL-treated group compared with the vehicle group and in the baicalin-treated group compared with the rPVL-treated group, respectively.

Article Snippet: NF2 (1:7000, Cat. No. 21686–1-AP), P-NF2 (Ser518, 1:7000, Cat. No. 28851–1-AP), CDK7 (1:2800, Cat. No. 27027–1-AP), and PKM (1:2800, Cat. No. 10078–2-AP) antibodies were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Histopathology, Infection, Control, Immunohistochemical staining, Expressing